Journal: Virulence

Article Title: MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

doi: 10.1080/21505594.2020.1735051

Figure Lengend Snippet: Sequences of primers, miRNAs, and siRNAs used in this study.

Article Snippet: The membrane was blocked with the polyclonal anti-Rab25 antibody (CSB-PA019175LA01HU, cusabio), and monoclonal anti-β-actin antibody (AB0035, Abways).

Techniques: Sequencing, Over Expression, Knockdown

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Changes in expression of G-proteins after 8-h stimulation by 10 n M DHT

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Rab25, Rab35 and AR expression in clinical samples. Rab25, Rab35 and AR protein expression levels were determined using immunohistochemical techniques for 154 epithelial cancer samples arrayed on a TMA. Representative sections are shown demonstrating predominantly cytoplasmic staining for Rab25 and Rab35, and nuclear staining for AR. Breast, thyroid and prostate sections were used as positive controls for Rab25, Rab35 and AR, respectively.

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Correlation of expression of Rab25 and Rab35 with clinical parameters

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: High expression of Rab25 predicts survival by histological type. ( A ) Kaplan–Meier survival curves of all patients included in this study show differential expression by histological type. ( B ) Kaplan–Meier curves of only those tumours showing low expression of Rab25 shows no difference in survival ( C ) Kaplan–Meier curves of only those tumours showing high expression of Rab25 attenuates this finding with improved survival in mucinous and endometrioid tumours compared with serous and clear cell cancers ( P =0.043).

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing, Quantitative Proteomics

Journal: Communications Biology

Article Title: Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits

doi: 10.1038/s42003-021-02934-0

Figure Lengend Snippet: List of Antibodies used in this paper.

Article Snippet: Purified anti-human RAB25 , Cell Signaling , 13048 T , 1:500.

Techniques: Flow Cytometry, Microscopy, Western Blot, Purification, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Cancer-specific cytotoxicity of curcumin through regulation of integrin β1 expression in colon cancer

doi: 10.1038/s41598-025-21627-w

Figure Lengend Snippet: After treatment of HCT116 cells with curcumin, an increase in integrin β1 and talin expression is observed, but no significant changes in rab25 expression are observed. ( a ) Expression of integrin β1, talin, and rab25, depending on curcumin treatment, is analyzed using western blotting; ( b ) Transcriptional levels of integrin β1, talin, and rab25 are measured using real-time polymerase chain reaction. Data were analyzed using the Mann–Whitney U test. Each condition was tested in triplicate across three independent experiments. Results are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. HCT human colon cancer cell line.

Article Snippet: The anti-integrin β1 (Cat: 34971), anti-rab25 (Cat: 13081), anti-talin 1 (Cat: 4021), anti-β-actin (Cat: 4970 S), HRP-linked anti-mouse IgG (Cat: 7076), and HRP-linked anti-rabbit IgG (Cat: 7074) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Cancer-specific cytotoxicity of curcumin through regulation of integrin β1 expression in colon cancer

doi: 10.1038/s41598-025-21627-w

Figure Lengend Snippet: Effects of rab25 knockdown and curcumin treatment on the expression of rab25, integrin β1, and talin in HCT 116 cells. ( a ) siRNA transfection effectively reduced rab25 expression. ( b ) Cell viability measured by MTT assay after sequential treatment with rab25 siRNA (20 nM) and curcumin (10 µg/mL). (c) Western blot analysis showing that rab25 knockdown transiently increased integrin β1 expression at 24 h, which was not sustained at 48 h. Curcumin treatment led to a marked upregulation of integrin β1 and talin expression at 48 h. ( d ) Transcriptional levels of rab25, integrin β1 and talin were measured using real-time PCR. Data were analyzed using the Mann–Whitney U test. Each condition was tested in triplicate across three independent experiments. Results are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. C, curcumin; HCT, human colon cancer cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; siRNA, small interfering ribonucleic acid.

Article Snippet: The anti-integrin β1 (Cat: 34971), anti-rab25 (Cat: 13081), anti-talin 1 (Cat: 4021), anti-β-actin (Cat: 4970 S), HRP-linked anti-mouse IgG (Cat: 7076), and HRP-linked anti-rabbit IgG (Cat: 7074) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Knockdown, Expressing, Transfection, MTT Assay, Western Blot, Real-time Polymerase Chain Reaction, MANN-WHITNEY

Journal: Oncology Research

Article Title: Knockdown of Ras-Related Protein 25 (Rab25) Inhibits the In Vitro Cytotoxicity and In Vivo Antitumor Activity of Human Glioblastoma Multiforme Cells

doi: 10.3727/096504016X14736286083065

Figure Lengend Snippet: Knockdown of Rab25 inhibits EMT phenotype in GBM cells. U87MG cells were transfected with sh-Rab25 or sh-NC for 48 h. (A) Western blot analysis was used to evaluate the protein levels of E-cadherin, N-cadherin, and vimentin. (B) Statistical analysis of E-cadherin, N-cadherin, and vimentin levels in the sh-NC and sh-Rab25 groups. * p < 0.05.

Article Snippet: The antibodies against Rab25, E-cadherin, N-cadherin, vimentin, p-PI3K, PI3K, p-AKT, AKT, and GAPDH were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Knockdown, Transfection, Western Blot